The Reds have named their team to play the Waratahs in Sydney with James Slipper captaining the team as James Horwill and Will Genia have both been ruled out.
Reds prop Slipper has been chosen as captain in his milestone 50th game for Queensland in the Templeton Cup contest.
Slipper, 24, regains the captaincy role he held during the opening month of the competition with Horwill (calf) and Genia (knee/hand) to miss one week through injury ahead of next weekend's Super Rugby play offs.
Slipper will become the fourth Reds player to reach the 50-game milestone for Queensland in 2013, joining Rob Simmons, Anthony Faingaa and James Hanson.
Slipper will pack down with prop Greg Holmes and hooker Saia Faingaa in the front-row while the absence of Horwill will see Ed O'Donoghue and Rob Simmons partner as the team's locks.
They'll be joined in the forwards by the young backrow trio of Eddie Quirk, Liam Gill and Jake Schatz.
Scrumhalf Nick Frisby will get his second straight opportunity to wear the number nine jersey with Genia sidelined for the weekend after Frisby starred in Queensland's historic tour match against The British and Irish Lions in June, scoring the team's second try.
He will combine with flyhalf Quade Cooper in the playmaking positions while goal-kicking ace Mike Harris has recovered from an ankle injury to win his first starting opportunity since round five and will join Ben Tapuai in the centres.
Ben Lucas retains his place at fullback as part of a back-three that includes flyers Rod Davies and Luke Morahan on the wings.
Reds Director of Coaching Ewen McKenzie has chosen a forward-heavy 5-2 split on the bench with Albert Anae, Jono Owen, Radike Samo, Jarrad Butler and Beau Robinson covering the forwards and Anthony Faingaa and Dom Shipperley in the backs.
For Owen it will be his first Super Rugby opportunity with the Reds although he has one Queensland cap from the team's thrilling encounter against The British and Irish Lions last month.
"We're in a unique circumstance where we've only got one game before the finals so it's important we begin to get our combinations right and are choosing players who are completely fit and healthy for the next stages of our campaign," McKenzie said.
"You never wish to see players of the calibre of James Horwill and Will Genia miss games but James Slipper did an outstanding job in the opening month of the season and will lead us well into what is a very important match against the Waratahs.
"Rivalries in Australian Rugby don't get much bigger and we're proud to have won the past four games against NSW. That's an important record to us, as is The Templeton Cup, and we'll be determined for that to continue."
Saturday night's encounter is crucial to the Reds' Super Rugby Finals Series ladder position with the Queenslanders locked in a battle for fourth place and a home Qualifying Final at Suncorp Stadium.
1. Greg Holmes,2. Saia Faingaa,3. James Slipper (c),4. Ed O'Donoghue,5. Rob Simmons,6. Eddie Quirk,7. Liam Gill,8. Jake Schatz,9. Nick Frisby,10. Quade Cooper (vc),11. Luke Morahan,12. Mike Harris,13. Ben Tapuai,14. Rod Davies,15. Ben Lucas
Replacements : 16. Albert Anae,17. Jono Owen,18. Radike Samo,19. Jarrad Butler,20. Beau Robinson,21. Anthony Faingaa,22. Dom Shipperley