The Cheetahs smashed the Lions 19-53 in Johannesburg with Riaan Viljoen scoring a hat-trick of tries in the Cheetahs bonus point victory.
The Lions losing streak now stands at 7 consecutive matches.
The Lions discipline and handling skills let them down again and the Cheetahs outclassed their opponents in just about every facet of the game.
The Cheetahs scored three tries in the first half and led the Lions 7-36 at half time.
After breaking the record last season for becoming Super rugby's worst ever performing team the Lions have now lost ten matches this season and won one.
The Lions sole victory this season was against the Cheetahs when they won 20-25 in Bloemfontein.
From the Lions last 25 Super Rugby matches against all opposition they have that one victory over the Cheetahs and there are no signs of potential improvement.
The result marked the second consecutive win for the men from Bloemfontein while their hosts will go on tour with seven straight losses behind them.
Although the score line may tell a different story, the Lions defence held up well in the opening stages of the match.
Some frantic tackling denied the visitors from scoring on three occasions.
The Cheetahs were content to take the numerous penalties on offer and on that front young Sias Ebersohn couldn't put a foot wrong all night.
As the match progressed the Lions defence -- for the first time this season -- started to resemble that of the team which went through the 2010 season without a win.
The loss also marked the two-year anniversary of the last time the Lions managed to secure a Super Rugby win at home.
Unlike in their previous two contest, the Cheetahs showed they can be a solid defensive unit, which will be important in their next fixture against the Crusaders in Bloemfontein.
One of the standout players for the visitors was flyhalf Ebersohn who kicked a perfect 10 out of 10.
Centre Corne Uys also shone with two tries, and the other three came courtsey of fullback Riaan Viljoen.
Skipper Adriaan Strauss again led from the front and showed why he is being considered to play for the Springboks in the World Cup later this year.
Another form of encouragement for the national coach was that flanker Heinrich Brussow lifted his performance another notch which means he could be back to his best form heading into the global showpiece in September.
The hosts fought back in the last 15 minutes of the match, but by then the Cheetahs had already sent on their replacements who had little more than defence practice against an inferior team.
Final Score Lions 19 Cheetahs 53
Tries - J Strauss, M. Rhodes, M. Bezuidenhout
Con - B Francis 2
Tries - C Uys , R Viljoen 3, R Ebersohn
Pen - S Ebersohn 6
Con - S Ebersohn 4, N Olivier
Referees: Craig Joubert (South Africa)
Assistant referees: Sindile Mayende (South Africa), Stefan Breytenbach (South Africa)
Television match official: Johann Meuwesen (South Africa)
Lions :Michael Killian, Lionel Mapoe, Dylan des Fontain, Doppies La Grange (capt), Deon van Rensburg, Burton Francis, Rory Kockott, Warren Whiteley, Josh Strauss, Cobus Grobbelaar, David de Villiers, Wikus van Heerden, Kevin Buys, Edgar Marutlulle, JC Janse van Rensburg.
Replacements: Martin Bezuidenhout, Patic Cilliers, Franco van der Merwe, Michael Rhodes, Jano Vermaak, Elton Jantjies, Jaco Taute
Cheetahs: 15 Riaan Viljoen, 14 Philip Burger, 13 Robert Ebersohn, 12 Corne Uys, 11 Riaan Smit, 10 Sias Ebersohn, 9 Sarel Pretorius, 8 Davon Raubenheimer, 7 Ashley Johnson, 6 Heinrich Brüssow, 5 Wilhelm Steenkamp, 4 Francois Uys, 3 WP Nel, 2 Adriaan Strauss (capt), 1 Coenie Oosthuizen.
Substitutes: 16 Ryno Barnes, 17 Lourens Adriaanse, 18 Waltie Vermeulen, 19 Kabamba Floors, 20 Tewis de Bruyn, 21 Naas Olivier, 22 Hennie Daniller.